All of the sera positive by the routine Western blot remained positive using the Scottish isolate antigen mix. When antigens from both isolates were incorporated into a single IgG Western blot, the results of a panel of sera were improved when compared to the routine reference strain IgG Western blot. burgdorferi sensu stricto) performed well, reproducing Western blot-positive results in 90 and 95% of tests, respectively. Two isolates, E5 (Borrelia afzelii) and G4 (B. burgdorferi sensu stricto antigen had different outcomes with these isolates. Sera previously found to be positive and negative when tested by routine Western blots prepared from reference strain B. Search HSWB or HSWBP code in the online Laboratory Test Catalog.Nine Scottish Borrelia burgdorferi isolates were investigated in IgG Western blot tests. For infants with suspected HSV disease, viral culture and PCR tests should be performed. HSV IgM antibody is present during both primary and recurrent infections making result interpretation difficult. This Western blot assay detects IgG antibody. Call Reference Laboratory Services if you have further questions, (800) 713-5198. If the antibody subtype is unclear (about 20% of specimens), the serum will be adsorbed against HSV-1 and HSV-2 proteins and re-tested. Most results are available within five days. HSV Western blot serologies are run three times a week. Since fewer than 5% of patients demonstrate a detectable rise in antibody titer during recurrent HSV episodes, an interpretive report is given instead of numerical values. In addition, Western blot readily documents seroconversion. The specificity of the Western blot assay provides an accurate distinction in 99% of patients between antibody to HSV-1 and antibodies to HSV-2. Our laboratory detects HSV Antibodies by Western blot assay, which is not only highly sensitive for detecting HSV antibodies, but also is highly accurate in differentiating past HSV-1 from HSV-2 infections and determining whether someone has antibodies to both viruses. However, serologies for HSV are useful in determining whether a person has had a past infection with HSV-1 or HSV-2, and is the best way to detect “silent carriers” of HSV-2. Viral isolation and subsequent subtyping is generally the best way to document an acute HSV infection. Search HBCA code in the online Laboratory Test Catalog for information on Hepatitis B Core Antibody (HBcAb). Search HBSA code in the online Laboratory Test Catalog for information on Hepatitis B Surface Antibody (HBsAb). Anti-HBs assays and anti-HBc assays are performed daily Monday through Friday. Vaccination/re-vaccination is warranted with values below this level. of Hepatitis B surface antibody are considered protective levels. Values above a test standard containing 10 I.U. The Hepatitis B surface antibody result is reported international units (I.U.) of Hepatitis B surface antibody. needle stick exposures), for documenting persons with past infection with Hepatitis B virus, for vaccine screening, and for determining response to vaccination. Hepatitis B surface antibody (anti-HBs) and Hepatitis B core antibody (anti-HBc) assays are useful for identifying persons susceptible to Hepatitis B infection (i.e. See Hepatitis B Chart 1, Chart 2, and Chart 3 Search HBB code in the online Laboratory Test Catalog for more information on Hepatitis B Battery (HBSAb, HBSAg, and HBCAb). Search HBSS code in the online Laboratory Test Catalog for more information on Hepatitis B Surface Antigen & Antibody (HBsAg, HBsAb). Search HBSAG or HBSAGX code in the online Laboratory Test Catalog. Note: Positive HBsAg will reflex to a Hepatitis B DNA by PCR at an additional charge. The physician must also report acute Hepatitis B infections to the local County Department of Public Health (in King County, telephone the Communicable Disease Department at (206) 296-4774). The routine HBsAg run can accommodate most specimens generated by accidental parenteral exposure. Patients with needle sticks and other parenteral exposures need to receive Hepatitis B Immune Globulin within 72 hours of exposure. Reports of reactive HBsAg are called to the ordering physician or laboratory and are automatically run for Hepatitis B viral DNA by PCR. Infectivity of a patient is determined by enzyme immunoassay for Hepatitis B surface antigen (HBsAg), which is run Monday through Friday. Hepatitis B is transmitted through blood or secretions of infected patients.
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